![]() The affinity of laccase at various enzyme concentration (0.3-0.9 mg mL -1), time (5 and 10 min) along with various substrates, i.e., 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), guaiacol (GCL), 2,6-dimethoxyphenol (DMP) and syringaldazine (SYZ), and its concentration (ABTS 0.5-1.5 mM, GCL 20-30 mM, DMP 1-5 mM, SYZ 10-30 mM) were inferred. In case of enzymatic profiling of blue laccase, its activity ranged from 0.04 to 464.3 U L -1 whereas in case of white laccase it ranged from 0.05 to 1404.7 U L -1. Our aim was to minimize its ambivalence, thus, total of five formulas (F1-F5) were used to determine laccase activity of white and blue laccase. However, there is ambivalence associated with various aspects of laccase, e.g., assay conditions and calculations. Laccase (benzenediol: oxygen oxidoreductase) a unique multi-copper oxidase enzyme has been studied rigorously since its identification.
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